lcw,25-Dihydroxyvitamin D,-Induced Upregulation of Calcineurin During Leukemic HL-60 Cell Differentiation
نویسنده
چکیده
Cyclosporin A and FK506, at concentrations that inhibited phosphatase activity of calcineurin in HL-60 cellular lysates, augmented the proliferation of leukemic HL-60 cells. These immunosuppressants did not affect Ia.25-dihydroxyvitamin D3 [1,25(0H),D,]-induced monocytic differentiation of HL-60 cells, but did abrogate the 1,25(OH)zD3-induced inhibition of HL-60 cell growth. Treatment with 20 nrnol/L 1,25(OH)2D3 led to a progressive increase in calcineurin phosphatase activity in subcellular fractions from HL-60 cell extracts; the increase in this activity appeared to parallel the phenotypic and functional changes of HL-60 cells during monocytic differentiation induced by 1,25(OH),D3. lmmunoblot analysis indicated that increase in calcineurin activity was concor-
منابع مشابه
اثر هم افزایی کاربرد توأم زهر زنبور عسل و25،1- دی هیدروکسی ویتامینD3 برالقای تمایز رده ی سلولی سرطانی پرومیلوسیتی HL-60
Introduction & Objective: Acute promyelocytic leukemia (APL) is a kind of acute leukemia characterized by a balanced t (15, 17) translocation which fails to develop into mature cells and proliferate in an unregulated fashion. In the recent years, in addition to combinatoral chemotherapy to treat unmature cancerous cells, differentiation therapy by differentiating agents as a novel procedure ...
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